The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F10. Next, Avidin...

ELISA Type: Sandwich. Detection Range: 0.63-40µg/mL. Sensitivity: 0.38µg/mL. Sample Types: Serum, plasma and other biological fluids. Protein function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting....

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

ELISA Type: CLIA. Sensitivity: 75 pg/mL. Range: 125--8000 pg/mL. Protein function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. [The UniProt Consortium]