The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EPX. Next,...

Sample Types: serum, plasma, tissue homogenates Detection Range: 0.156 ng/mL-10 ng/mL Sensitivity: 0.039 ng/mL Assay Principle: quantitative Measurement: SandwichAssay Time: 1-5h Sample Volume: 50-100ulDetection Wavelength: 450 nmProtein function: Mediates tyrosine nitration of secondary granule proteins in mature...

ELISA Type: Sandwich ELISA, Double Antibody. Detection Range: 15.6-1000µg/mL. Sensitivity: 9.375µg/mL. Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples. Protein function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. [The...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Eosinophil Peroxidase (EPX) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPX in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 31.2-2000pg/ml, Sensitivity: 12.7pg/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit...