The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCK1. Next,...

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids,...

Activity: Testing in progress Protein Construction: A DNA sequence encoding the Rat PEPCK-C protein (P07379) (Met 1-Lys 135) was expressed with a N-His tag. Sequence: Met 1-Lys 135. Fusion tag: N-His Endotoxin: Please contact us for more information. Apparent Molecular Mass: 15 kDa. Protein function: PCK1...

Protein function: Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed:26322521, PubMed:26709450, PubMed:28345895, PubMed:30193097, PubMed:31461616, PubMed:4186849)....

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....