Description: The enzyme BirA is a key reagent because of its ability to biotinylate proteins at a specific residue in a recognition sequence. This enzyme is used to biotinylate the C termini of membrane proteins, allowing these proteins to be tetramerized by binding to streptavidin. Because of the specificity of the...

BirA-transformed Competent E. coli cells are supplied as 10 x 100 µl vials. Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene. These cells are compatible with most cloning vectors and are...

Protein function: Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio- 5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein...

Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal GST-tag, MW = 61, expressed in an E. coli cell expression system. Protein function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also...

The In Vitro Biotinylation Kit (with BirA Tagless Enzyme) is a kit designed for in vitro addition of biotin to biotin-acceptor peptides using BirA. This kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin. The BirA protein provided is...