The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CAPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CAPN1. Next,...

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-713aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MTEELITPVY CTGVSAQVQK KRDKELGLGR HENAIKYLGQ DYETLRARCL QSGVLFQDEA FPPVSHSLGF KELGPHSSKT YGIKWKRPTE LMSNPQFIVD GATRTDICQG ALGDCWLLAA IASLTLNETI...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Calpain 1, Large Subunit (CAPN1) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAPN1 in mouse tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.049ng/ml, Storage and Stability: Desiccation recommended. The...

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Source: Full length, recombinant protein corresponding to aa1-713 from mouse Capn1, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight:...