This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene,along with GTP,catalyzes the formation of phosphoenolpyruvate from oxaloacetate,with the release of carbon dioxide and GDP. The expression of this gene can be regulated by...

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCK1. Next,...

PCK1 (Phosphoenolpyruvate carboxykinase 1, also PEPCK-C [cytosolic]) is a monomeric, 67-68 kDa member of the PEP carboxykinase family of enzymes. It is expressed in postnatal cells such as mammary epithelium, white and brown adipocytes, skeletal muscle cells and hepatocytes. PCK1 has multiple functions, some of...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....