Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 3747-4015aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EYPFLVKGGE DLRQDQRIEQ IFEVMNAILS QDAACSQRNM QLRTYRVVPM TSRLGLIEWI ENTMTLKDLL LSNMSQEEKV ANNSDPKAPI RDYKDWLMKV SGKSDAGAYV LMYSRANRTE TVVAFRRRES...

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRKDC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRKDC. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRKDC in mouse tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.105ng/ml, Storage and Stability: Desiccation...

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J...