Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-637aa. Protein Length: Full Length. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: MGLLSQGSPL SWEETQRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE VEYMLVSFDH ENRKVQLLLN GGDVLETLQE KGERTNPNHP TLWRPEYGSY MIEGTPGQPY GGTMSEFNTV EANMRKRRKE...

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCLC. Next,...

ELISA Type: Sandwich ELISA, Double Antibody. Detection Range: 31.25-2000µg/mL. Sensitivity: 18.75µg/mL. Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples. Protein function: Catalyzes the ATP-dependent ligation of L-glutamate and L- cysteine and participates in the...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Glutamate Cysteine Ligase, Catalytic (GCLC) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GCLC in mouse serum, plasma and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.118ng/ml, Storage and Stability: Desiccation recommended....