ELISA Type: CLIA. Sensitivity: 4.688 pg/mL. Range: 7.813--500 pg/mL. Protein function: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. [The UniProt Consortium]

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GHRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GHRH. Next, Avidin...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

The Mouse Growth Hormone Releasing Hormone (GHRH) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of GHRH in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 12.35-1,000pg/ml, Sensitivity:...