Description: 5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Target: Others. Smiles:...

5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE (Cay-14456), 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used...

Dark orange to brown solid. Soluble in DMSO. Carboxyfluorescein is commonly employed as a polar tracer and used for intracellular pH measurements. It has also been used for studying viability of various types of bacteria.

Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the...