To stimulate mouse bone marrow-derived macrophages, Cord Factor was suspended at a concentration of 0.2 mg/ml in hexane. Of the resulting solution 1 or 10 µg/well were layered in 96-well tissue culture plates and the solvent completely evaporated. Control wells were layered with solvent without Cord Factor and also...

6,6'-Trehalose Dimycolate (Cord Factor, source: Mycobacterium bovis, Bacillus Calmette Guérin) 6,6'-dimycoloyl-alpha-D-trehalose (Mycobacterium bovis, BCG) is used as an immunoadjuvant for the induction and non-specific stimulation of antibody production against unrelated antigens. This results in an increased host...