Description: NSC 185058, an inhibitor of ATG4B (a major cysteine protease), can also attenuate autophagic activity. Target: Cysteine Protease, Autophagy. Smiles: S=C(Nc1ccccn1)c1ccccn1. References: Akin D, et al. A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors....

NSC 185058 is an inhibitor of ATG4B, a cysteine protease that activates LC3B during the initiation of autophagy. It inhibits ATG4B cleavage of LC3B-GST (IC50 = 51 µM) and reduces LC3B lipidation induced by starvation in Saos-2 cells. NSC 185058 (100 µM) reduces starvation-induced autophagy and prevents autophagic...