MTT, a yellow tetrazole, is reduced to purple formazan in living cells. A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a...

Description: Thiazolyl Blue (MTT) is a colorimetric compound extensively utilized to measure cell proliferation, transitioning from a yellow color to purple formazan in living cells. Target: Others. Smiles: CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]. References: Berridge MV, et al. Tetrazolium dyes as...

MTT is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. It is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD(P)H-oxidoreductases. It is frequently used in colorimetric assays to measure cell proliferation,...

Yellow powder. Soluble in water (5mg/ml). Chemical. CAS: 298-93-1. Formula: C18H16BrN5S. MW: 414.32. MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular...

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for viability, proliferation and cytotoxicity assays. On reduction tetrazolium salts turn into deeply colored products that can be easily quantified by colorimetric...