Solid. Soluble in DMSO, DMF or acetone. Widely used fluorophore, well-known for preparing fluorogenic substrates for cystine aminopeptidase and other hydrolases. Used as reference compound in enzyme assays.

Liquid. A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be calculated from a standard curve prepared with AMC standards.

7-Amino-4-methylcoumarin (AMC) is a fluorescent probe for peptide labeling commonly used in the study of proteases. Conjugation of AMC via its amino group to a peptide substrate results in quenching of the fluorescent signal. Upon enzymatic cleavage of the peptide by proteases, AMC is released and its fluorescence...

This compound is the precursor for a variety of AMC enzyme substrates. It is also used as a calibration standard for AMC-based enzyme substrates. Fluorogenic AMC substrates are widely used for measuring a variety of protease activities.

Liquid. Chemical. CAS: 26093-31-2. Formula: C10H9NO2. MW: 175.2. Synthetic. A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be...