Description: Calcein Blue AM is a live cell labeling dye that permeates the cell membrane. Upon entering cells, intracellular esterases cleave the ester group of acetoxymethyl (AM), producing Calcein Blue fluorescent dye that is impermeable to cell membranes. Smiles:...

It is a cell-permeable version of calcein blue. Upon getting into live cells the weakly fluorescent calcein blue AM is hydrolyzed into calcein blue that has the excitation/emission maxima similar to those of DAPI, Hoechst and AMCA. This exceptional spectral separation from the typical green and red fluorophores...

Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue (Cay-20636) by intracellular esterases. Calcein Blue is retained by living cells and displays...