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CFDA-SE is a stable, cell-permeable dye that consists of a fluorescein molecule containing two acetate moieties and a succinimidyl ester (SE) functional group. Upon diffusion into the intracellular environment, the acetate groups are cleaved by cellular esterases leaving CFSE (Cay-16802), which is fluorescent and...

Description: CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm....

CFDA, SE is an amine reactive FDA derivative that can be used to prepare a variety of FDA conjugates.

Non-fluorescent and moderately hydrophobic in its di-esterified form, 5,6 CFDA,SE easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CFSE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is...

White or slightly yellow powder. Soluble in DMSO or DMF. 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent...

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives...