Description: 5-CFDA-AM is a cell-permeable esterase substrate used as an active probe to evaluate enzyme activity and cell membrane integrity. Electroneutral and able to enter cells at lower concentrations than CFDA, it undergoes intracellular hydrolysis by esterases to produce carboxyfluorescein, which possesses an...

5-Carboxyfluorescein diacetate, acetoxymethyl ester (CFDA, AM) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their...