Naveni PD1/PDL1 BOND RX Atto647

The Naveni(R) PD1/PD-L1 BOND RX Atto647N is an in situ proximity ligation assay kit for the detection of the interaction of PD1 and PD-L1 using BOND RX/RXm Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples. Not recommend for mouse tissue. For research use only. Not for use in diagnostic procedures. The kit is based on the Naveni(R) in situ proximity ligation technology with automated workflow. It uses two Navenibodies linked to proprietary oligo arms. The primary antibodies included in the kit bind to PD1 and PD-L1 proteins, while Navenibodies then bind to their respective primary antibodies. When Navenibodies are in close proximity, a strong and distinct signal is generated, offering a specific and sensitive detection method for the interaction between PD1 and PD-L1. Included in the Naveni(R) PD1/PD-L1 BOND RX Atto647N kit: Navenibody targeting human PD1 protein based on clone EH33 CST Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb(R) Buffers for blocking and dilutions and detection reagents for the PD1/PD-L1 interaction signal Reagents sufficient for 30 FFPE tissue slides, including dead volumes. PD1 Protein function: Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. [The UniProt Consortium] PDL1 Protein function: Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). [The UniProt Consortium]