ReadiLink(TM) iFluor(TM) 555 Protein Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 555 dyes have fluorescence excitation and emission maxima of 550 nm and 570 nm respectively. iFluor(TM) 555 family has the spectral properties essentially identical to those of Cy3(R) (Cy3(R) is the trademark of GE Healthcare). Compared to Cy3 probes iFluor(TM) 555 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy3(R). iFluor(TM) 555 family has become an excellent replacement to Cy3 and Alexa Fluor(R) 555 labeling dye (Alexa Fluor(R) is the trademark of Invitrogen). iFluor(TM) 555 SE used in the ReadiLink(TM) kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components for labeling about 100 ug antibody. iFluor(TM) 555 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor(TM) 555 SE.