iFluor(R) 570 succinimidyl ester

Excitation (nm): 560. Emission (nm): 571. Quantum yield: 0.58. AAT Bioquest's iFluor(R) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(R) 570 family has the spectral properties essentially identical to those of Cy3B. It is a superior replacement to Cy3B with greatly improved water solubility compared to Cy3B that is barely soluble in aqueous solutions. It maintains other charactuers of Cy3B, e.g., high fluorescence quantum yield with greatly enhanced photostability. iFluor(R) 570 family has fluorescence that is pH-independent from pH 3 to 11. iFluor(R) 570 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.