Buccutite(TM) Rapid PE Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per

Ex: 565 nm. Em: 575 nm. Soluble in: DMSO. RPE is an orange fluorescent protein which has an excitation wavelength of 565nm and an emission wavelength of 575 nm. Buccutite(TM) RPE Conjugation Kit provides a simple and convenient method to conjugate your antibodies with RPE. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 50 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of RPE. The best ratio for any new antibody reagent must be determined by experimentation but 50-60 ug of IgG antibody for every 100 ug of RPE usually gives optimal results. 60 ug of antibody approximately corresponds to an Antibody:RPE molar ratio of 1:1.