Y-box-binding protein 3 (YBX3), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-372aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SEAGEATTTT TTTLPQAPTE AAAAAPQDPA PKSPVGSGAP QAAAPAPAAH VAGNPGGDAA PAATGTAAAA SLATAAGSED AEKKVLATKV LGTVKWFNVR NGYGFINRND TKEDVFVHQT AIKKNNPRKY LRSVGDGETV EFDVVEGEKG AEAANVTGPD GVPVEGSRYA ADRRRYRRGY YGRRRGPPRN YAGEEEEEGS GSSEGFDPPA TDRQFSGARN QLRRPQYRPQ YRQRRFPPYH VGQTFDRRSR VLPHPNRIQA GEIGEMKDGV PEGAQLQGPV HRNPTYRPRY RSRGPPRPRP APAVGEAEDK ENQQATSGPN QPSVRRGYRR PYNYRRRPRP PNAPSQDGKE AKAGEAPTEN PAPPTQQSSA E. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full-length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression. Reference: n/a. Function: nan