Voltage-dependent L-type calcium channel subunit alpha-1D (Cacna1d), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1644-2144aa. Protein Length: Partial. Tag Info: C-terminal GFP-tagged. Target Protein Sequence: EQGLVGKYPA KNTTIALQAG LRTLHDIGPE IRRAISCDLQ DDEPEDSKPE EEDVFKRNGA LLGNHVNHVN SDRRDSLQQT NTTHRPLHVQ RPSMPPASDT EKPLFPPAGN SGCHNHHNHN SIGKQAPTST NANLNNANMS KAAHGKPPSI GNLEHVSENG HYSCKHDREL QRRSSIKRTR YYETYIRSES GDEQFPTICR EDPEIHGYFR DPRCLGEQEY FSSEECCEDD SSPTWSRQNY NYYNRYPGSS MDFERPRGYH HPQGFLEDDD SPTGYDSRRS PRRRLLPPTP PSHRRSSFNF ECLRRQSSQD DVLPSPALPH RAALPLHLMQ QQIMAVAGLD SSKAQKYSPS HSTRSWATPP ATPPYRDWSP CYTPLIQVDR SESMDQVNGS LPSLHRSSWY TDEPDISYRT FTPASLTVPS SFRNKNSDKQ RSADSLVEAV LISEGLGRYA RDPKFVSATK HEIADACDLT IDEMESAAST LLNGSVCPRA NGDMGPISHR Q. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Reference: n/a. Function: nan