Voltage-dependent L-type calcium channel subunit alpha-1C (CACNA1C), partial, guinea pig, recombinan

Organism: Cavia porcellus (Guinea pig). Source: in vitro E.coli expression system. Expression Region: 1-169aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: FQEQGEQEYK NCELDKNQRQ CVEYALKARP LRRYIPISIT FFRLFRVMRL VKLLSRGEGI RTLLWTFIKS FQALPYVALL IVMLFFIYAV IGMQVFGKIA LNDTTEINRN NNFQTFPQAV LLLFRCATGE AWQDIMLACM PGKKRAPESE PSNSTEGETP CGSSFAVFY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (By similarity). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (By similarity). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (By similarity). Activity regulation Inhibited by dihydropyridines (DHP), such as isradipine. Inhibited by nifedipine. Channel activity is regulated by Ca2+ and calmodulin. Binding of STAC1, STAC2 or STAC3 to a region that overlaps with the calmodulin binding site inhibits channel inactivation by Ca2+ and calmodulin (By similarity). Binding of calmodulin or CABP1 at the same regulatory sites results in opposite effects on the channel function. Shear stress and pressure increases calcium channel activity (By similarity). Reference: "Gestational expression of voltage-dependent calcium channel subunits in guinea pig uterus." Collins P.L., Lundgren D.W., Kulp T.M., Shah P., Chang S.M., Chang A.S. Submitted (MAY-1997). Function: nan