Vitronectin (Vtn), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 20-478aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: DQESCKGRCT QGFMASKKCQ CDELCTYYQS CCADYMEQCK PQVTRGDVFT MPEDDYWSYD YVEEPKNNTN TGVQPENTSP PGDLNPRTDG TLKPTAFLDP EEQPSTPAPK VEQQEEILRP DTTDQGTPEF PEEELCSGKP FDAFTDLKNG SLFAFRGQYC YELDETAVRP GYPKLIQDVW GIEGPIDAAF TRINCQGKTY LFKGSQYWRF EDGVLDPGYP RNISEGFSGI PDNVDAAFAL PAHRYSGRER VYFFKGKQYW EYEFQQQPSQ EECEGSSLSA VFEHFALLQR DSWENIFELL FWGRSSDGAR EPQFISRNWH GVPGKVDAAM AGRIYVTGSL SHSAQAKKQK SKRRSRKRYR SRRGRGHRRS QSSNSRRSSR SIWFSLFSSE ESGLGTYNNY DYDMDWLVPA TCEPIQSVYF FSGDKYYRVN LRTRRVDSVN PPYPRSIAQY WLGCPTSEK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Reference: "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005). Function: nan