Vascular cell adhesion protein 1 (Vcam1), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 31-697aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: PEYKTLAQIG DSMLLTCSTT GCESPSFSWR TQIDSPLNGK VKTEGAKSVL TMDPVSFENE HSYLCTATCN SGKLERGIQV DIYSFPKDPE IQFSGPLEVG KPVMVKCLAP DVYPIDRLEI ELFKGDRLMK KQDFVDEMAK KSLETKSLEV IFTPVIEDIE KALVCRAKLY IDQTDSIPKE RETVRELQVY TSPKNTEISV HPSTRLHEGA AVTMTCASEG LPAPEIFWSK KLDNGVLQLL SGNATLTLIA MRMEDSGIYV CEGVNLVGRD KTEVELIVQE KPFTVDISPG SQVAAQVGDS VVLTCAAVGC DSPSFSWRTQ TDSPLNGEVR DEGATSTLTL SPVGVEDEHS YLCTVTCQRR KLEKTIQVEV YSFPEDPEIE ISGPLVHGRP VTVNCTVPNV YPFDHLEIEL LKGETTLLNK FLREEIGTKS LETKSLEMTF IPTAEDTGKA LVCLAKLHSS QMESEPKQRQ STQTLYVNVA PKEPTIWVSP SPVPEEGSPV NLTCSSDGFP TPKILWSRQL KNGELQPLSQ NTTLSFMATK MEDSGIYVCE GINEAGISKK SVELIIQGSS KDIQLTVFPS KSVKEGDTVI ISCTCGSVPE IWIILKKKAK TGDMVLKSVN GSYTIRKAQL QDAGVYECES KTEVGSQLRS LTLDVKGKEN NKDYFSP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte igration to sites of inflammation. Reference: Cloning of murine and rat vascular cell adhesion molecule-1.Hession C., Moy P., Tizard R., Chisholm P., Williams C., Wysk M., Burkly L., Miyake K., Kincade P., Lobb R.Biochem. Biophys. Res. Commun. 183:163-169(1992). Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.