Tryptase (Tpsab1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 35-267aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AHGNKWPWQV SLRANDTYWM HFCGGSLIHP QWVLTAAHCV GPDVADPNKV RVQLRKQYLY YHDHLMTVSQ IITHPDFYIV QDGADIALLK LTNPVNISDY VHPVPLPPAS ETFPSGTLCW VTGWGNIDNG VNLPPPFPLK EVQVPIIENH LCDLKYHKGL ITGDNVHIVR DDMLCAGNEG HDSCQGDSGG PLVCKVEDTW LQAGVVSWGE GCAQPNRPGI YTRVTYYLDW IHH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Reference: Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.McNeil H.P., Reynolds D.S., Schiller V., Ghildyal N., Gurley D.S., Austen K.F., Stevens R.L.Proc. Natl. Acad. Sci. U.S.A. 89:11174-11178(1992). Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).