Transmembrane protease serine 4 (TMPRSS4), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 54-437aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: KVILDKYYFL CGQPLHFIPR KQLCDGELDC PLGEDEEHCV KSFPEGPAVA VRLSKDRSTL QVLDSATGNW FSACFDNFTE ALAETACRQM GYSSKPTFRA VEIGPDQDLD VVEITENSQE LRMRNSSGPC LSGSLVSLHC LACGKSLKTP RVVGVEEASV DSWPWQVSIQ YDKQHVCGGS ILDPHWVLTA AHCFRKHTDV FNWKVRAGSD KLGSFPSLAV AKIIIIEFNP MYPKDNDIAL MKLQFPLTFS GTVRPICLPF FDEELTPATP LWIIGWGFTK QNGGKMSDIL LQASVQVIDS TRCNADDAYQ GEVTEKMMCA GIPEGGVDTC QGDSGGPLMY QSDQWHVVGI VSWGYGCGGP STPGVYTKVS AYLNWIYNVW KAEL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plasma membrane-anchored serine protease that directly induces processing of pro-uPA/PLAU into the active form through proteolytic activity. Seems to be capable of activating ENaC. , (Microbial infection) In gut epithelial cells, facilitates human coronavirus SARS-CoV-2 infection through, at least, the cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. Reference: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M. Genome Res. 13:2265-2270(2003). Function: nan