Transmembrane protease serine 2 (TMPRSS2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 106-492aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: WKFMGSKCSN SGIECDSSGT CINPSNWCDG VSHCPGGEDE NRCVRLYGPN FILQVYSSQR KSWHPVCQDD WNENYGRAAC RDMGYKNNFY SSQGIVDDSG STSFMKLNTS AGNVDIYKKL YHSDACSSKA VVSLRCIACG VNLNSSRQSR IVGGESALPG AWPWQVSLHV QNVHVCGGSI ITPEWIVTAA HCVEKPLNNP WHWTAFAGIL RQSFMFYGAG YQVEKVISHP NYDSKTKNND IALMKLQKPL TFNDLVKPVC LPNPGMMLQP EQLCWISGWG ATEEKGKTSE VLNAAKVLLI ETQRCNSRYV YDNLITPAMI CAGFLQGNVD SCQGDSGGPL VTSKNNIWWL IGDTSWGSGC AKAYRPGVYG NVMVFTDWIY RQMRADG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed:25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed:15537383, PubMed:26018085, PubMed:25122198). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity). Reference: "The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells." Wilson S., Greer B., Hooper J., Zijlstra A., Walker B., Quigley J., Hawthorne S. Biochem. J. 388:967-972(2005). Function: nan