Tissue Factor (TF) Recombinant, Rabbit

Recombinant rabbit tissue factor is supplied as a ~46kD molecule. This apoprotein contains extra cellular and transmembrane domains of the tissue factor, plus an amino terminal leader sequence consisting of several domains which aid in expression and purification. Application: Human tissue factor has been cloned and expressed in both E. coli and Baculovirus expression systems and successfully configured into very sensitive thromboplastin reagents. However, these reagents have shown to be sensitive to Proteins Induced by Vitamin K Antagonism or Absence (PIVKAs) in patient clinical samples which can detrimentally affect laboratory test results. Thus a project was undertaken to clone and express rabbit tissue factor to overcome the PIVKA sensitivity and create a PT reagent raw material which builds upon the vast experience of using rabbit derived thromboplastins in the clinical laboratory setting. Using the published cDNA gene sequence for rabbit brain tissue factor, the gene was isolated and inserted into a prokaryotic expression system. The protein was expressed as a fusion protein to increase both the solubility and the biological activity of the rRTF molecule. The result is a protein with a molecular weight of approximately 45kD including the 12kD fusion partner. After lipidation with synthetic phospholipids or phospholipids purified from rabbit brain, the rRTF has functional prothrombin activity in a normal prothrombin clotting assay. Cleavage of the fusion protein did not increase the activity of the molecule. Thus, a thromboplastin reagent made with rRTF may be useful as a raw material for a clinical prothrombin reagent. Prothrombin Time: Citrol Level 1: 9.2 seconds ± 1.0 second , Protein Purity (SDS-PAGE):, 80% ± 10%, Protein Concentration: 240 ± 15ug/ml, Storage: Product quality is guaranteed for 1 year from the date of receipt by the customer as long as the product is stored in accordance with the indicated storage conditions.