Testis-specific serine/threonine-protein kinase 3 (TSSK3), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-268aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKV IDKMGGPEEF IQRFLPRELQ IVRTLDHKNI IQVYEMLESA DGKICLVMEL AEGGDVFDCV LNGGPLPESR AKALFRQMVE AIRYCHGCGV AHRDLKCENA LLQGFNLKLT DFGFAKVLPK SHRELSQTFC GSTAYAAPEV LQGIPHDSKK GDVWSMGVVL YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLSISADCQD LLKRLLEPDM ILRPSIEEVS WHPWLAST. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in a signaling pathway during male germ cell development or mature sperm function. Reference: "Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling." Bucko-Justyna M., Lipinski L., Burgering B.M., Trzeciak L. FEBS J. 272:6310-6323(2005). Function: May be involved in a signaling pathway during male germ cell development or mature sperm function.