TEAD4 Cofactor-Binding Domain (human, recombinant)

TEA domain transcription factor 4 (TEAD4) is a transcription factor and member of the TEA domain-containing family of transcription factors. It is composed of an N-terminal TEA DNA-binding domain, a hydrophobic proline-rich domain, and a C-terminal cofactor-binding domain (CBD), which binds tafazzin (TAZ), YES-associated transcriptional regulator (YAP), or vestigial-like proteins (VGLLs), and is involved in TAZ-, YAP-, or VGLL-dependent transcription of genes in the Hippo signaling pathway, which regulates embryonic and organ development, proliferation, and cell death. TEAD4 associates with VGLL3 to regulate growth and differentiation in primary mouse myotubes, but not primary mouse myoblasts, as well as with VGLL1, TAZ, or YAP to promote transcription of cell cycle and self-renewal genes and induce a trophectoderm lineage in human pluripotent stem cells (PSCs). The tertiary structure and function of TEAD4 requires C-terminal palmitoylation at residue 360. A CBD-only TEAD4 isoform binds TEAD cofactors and acts as a negative regulator of Hippo signaling, which decreases proliferation and the levels of epithelial-to-mesenchymal transition (EMT) proteins when expressed in cancer cells. Increased tumor levels of TEAD4 are associated with decreased survival time in patients with lung adenocarcinoma (LUAD), but increased levels of the CBD-only TEAD4 isoform in tumors are associated with increased survival time in patients with LUAD or colon cancer. Cayman's TEAD4 Cofactor-Binding Domain (human, recombinant) protein consists of 219 amino acids, has a calculated molecular weight of 25.5 kDa, and is depalmitoylated at cysteine 360.Synonyms: EFTR-2, Embroyonic TEA Domain-containing Factor 2, RTEF-1, TEA Domain Transcription Factor 4, TEF-3, Transcriptional Enhancer Factor 3. Source: Recombinant human TEAD4 cofactor-binding domain expressed in insect cells. Amino Acids: 217-434. MW: 25.5 kDa. UniProt Accession No: Q15561.