Taste receptor type 2 member 13 (TAS2R13), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 1-303aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MESALPSIFT LVIIAEFIIG NLSNGFIVLI NCIDWVSKRE LSSVDKLLII LAISRIGLIW EILVSWFLAL HYLAIFVSGT GLRIMIFSWI VSNHFNLWLA TIFSIFYLLK IASFSSPAFL YLKWRVNKVI LMILLGTLVF LFLNLIQINM HIKDWLDRYE RNTTWNFSMS DFETFSVSVK FTMTMFSLTP FTVAFISFLL LIFSLQKHLQ KMQLNYKGHR DPRTKVHTNA LKIVISFLLF YASFFLCVLI SWISELYQNT VIYMLCETIG VFSPSSHSFL LILGNAKLRQ AFLLVAAKVW AKR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Reference: "Role of the G-protein subunit alpha-gustducin in taste cell responses to bitter stimuli." Caicedo A., Pereira E., Margolskee R.F., Roper S.D. J. Neurosci. 23:9947-9952(2003). Function: nan