T7 RNA Polymerase, GMP-Grade DMF #037660

Product Description: T7 RNA Polymerase (T7RNAP) is the key enzyme for in vitro transcription (IVT) of mRNA. T7 Polymerase is a DNA-dependent polymerase that efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7RNAP initiates transcription at the G* in the classic T7 promoter sequence-taatacgactcactataG*GG. T7 Polymerase is highly specific to its promoter sequence. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended. Note: GMP T7 RNA Polymerase requires the cofactor Mg2+ for mRNA synthesis. We recommend restriction enzymes that produce blunt ends or 5'-overhangs. We've filed our GMP T7RNAP with the FDA Drug Master Files (DMF) and its been assigned DMF #037660. Application: mRNA Synthesis, in vitro transcription (IVT), RNA analysi,s Radiolabeled RNA probe preparation, Hybridization, RNase protection assay. Concentration: 50U/µL. Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50µL in 1 hour at 37°C. Source: Expressed in an E.coli strain that carries the T7 RNA Polymerase gene. Quality Standards: Activity (Molecular Beacon): > 50 kU/mL, Purity (SEC-HPLC): > 95%, Residual DNase: Negative, Residual RNase: Negative, Residual Protease: Negative, Endotoxin: <= 10 EU/mL ,Residual Host Cell DNA: <= 100 pg/mg, Residual Host Protein: <= 20 ng/mg, Residual Heavy Metal: <= 10ppm, Residual Nickel Salt: <= 10 ppm, Bioburden: <= 1 CFU/10mL.