Squalene monooxygenase (SQLE), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 42-574aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LSYRCRHRNG GLLGRQQSGS QFALFSDILS GLPFIGFFWA KSPPESENKE QLEARRRRKG TNISETSLIG TAACTSTSSQ NDPEVIIVGA GVLGSALAAV LSRDGRKVTV IERDLKEPDR IVGEFLQPGG YHVLKDLGLG DTVEGLDAQV VNGYMIHDQE SKSEVQIPYP LSENNQVQSG RAFHHGRFIM SLRKAAMAEP NAKFIEGVVL QLLEEDDVVM GVQYKDKETG DIKELHAPLT VVADGLFSKF RKSLVSNKVS VSSHFVGFLM KNAPQFKANH AELILANPSP VLIYQISSSE TRVLVDIRGE MPRNLREYMV EKIYPQIPDH LKEPFLEATD NSHLRSMPAS FLPPSSVKKR GVLLLGDAYN MRHPLTGGGM TVAFKDIKLW RKLLKGIPDL YDDAAIFEAK KSFYWARKTS HSFVVNILAQ ALYELFSATD DSLHQLRKAC FLYFKLGGEC VAGPVGLLSV LSPNPLVLIG HFFAVAIYAV YFCFKSEPWI TKPRALLSSG AVLYKACSVI FPLIYSEMKY MVH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis. Reference: "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004). Function: nan