Spike glycoprotein (S), partial (Active), Severe acute respiratory syndrome coronavirus 2, recombina

Organism: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2). Source: Mammalian cell. Expression Region: 319-541aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml. ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml. ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 µg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 2.785-9.139 ng/ml. ?SARS-CoV-2 Spike protein RBD his/myc tag (CSB-MP3324GMY1b1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 13.8 nM as detected by LSPR Assay. ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind Biotinylated human ACE2 (CSB-MP866317HU-B), the EC50 is 4.599-8.322 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan