SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2)

SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S5455-04H.10 10 µg - -

3 - 19 business days*

1,045.00€
 
Human Sphingosine kinase 2 ( GenBank Accession No.NM_020126), full length with N-terminal His... more
Product information "SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2)"
Human Sphingosine kinase 2 ( GenBank Accession No.NM_020126), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay Buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.75ug/ml sphingosine kinase 2. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Keywords: SK 2, SPK 2, EC=2.7.1.91
Supplier: United States Biological
Supplier-Nr: S5455-04H

Properties

Application: Enzyme assays
Conjugate: No
Format: Purified

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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