Serine/threonine-protein phosphatase 5 (PPP5C), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-499aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AMAEGERTEC AEPPRDEPPA DGALKRAEEL KTQANDYFKA KDYENAIKFY SQAIELNPSN AIYYGNRSLA YLRTECYGYA LGDATRAIEL DKKYIKGYYR RAASNMALGK FRAALRDYET VVKVKPHDKD AKMKYQECNK IVKQKAFERA IAGDEHKRSV VDSLDIESMT IEDEYSGPKL EDGKVTISFM KELMQWYKDQ KKLHRKCAYQ ILVQVKEVLS KLSTLVETTL KETEKITVCG DTHGQFYDLL NIFELNGLPS ETNPYIFNGD FVDRGSFSVE VILTLFGFKL LYPDHFHLLR GNHETDNMNQ IYGFEGEVKA KYTAQMYELF SEVFEWLPLA QCINGKVLIM HGGLFSEDGV TLDDIRKIER NRQPPDSGPM CDLLWSDPQP QNGRSISKRG VSCQFGPDVT KAFLEENNLD YIIRSHEVKA EGYEVAHGGR CVTVFSAPNY CDQMGNKASY IHLQGSDLRP QFHQFTAVPH PNVKPMAYAN TLLQLGMM. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT . Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses . Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1 . Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress . Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function . Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1 . Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E . May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels . Dephosphorylates and may play a role in the regulation of TAU/MAPT . Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2 . Dephosphorylate FNIP1, disrupting interaction with HSP90AA1/Hsp90 . Reference: "The Kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5." Sekine Y., Hatanaka R., Watanabe T., Sono N., Iemura S., Natsume T., Kuranaga E., Miura M., Takeda K., Ichijo H. Mol. Cell 48:692-704(2012). Function: nan