SARS-CoV-2 Nucleocapsid Protein

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV-2 RNA. SARS-CoV-2 is a member of the Betacoronavirus genus of viruses and has 88% sequence identity with two bat-derived SARS-like CoVs. SARS-CoV-2 nucleocapsid protein is composed of an N-terminal domain (NTD) that binds RNA, a disordered linker region that contains regulatory phosphorylation sites, a C-terminal domain (CTD) that mediates dimerization, and a C-terminal tail. In a similar virus, SARS-CoV, the nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein (RNP) complex that is a template for viral replication. The SARS-CoV nucleocapsid protein is integral for viral self-assembly and is involved in cell cycle regulation. The SARS-CoV-2 nucleocapsid protein gene sequence is greater than 90% similar to the SARS-CoV nucleocapsid protein, and it contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes. SARS-CoV-2 nucleocapsid protein inhibits the formation of stress granules, which sequester viral factors and are involved in the host antiviral response. SARS-CoV-2 is the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. Cayman's SARS-CoV-2 Nucleocapsid Protein can be used as an antigen or for Western blot (WB), ELISA, protein-protein interaction studies, and other in vitro binding and in vivo functional assays.Synonyms: 2019-nCoV NP, 2019-nCoV Nucleocapsid Protein, 2019-nCoV Nucleoprotein, COVID-19 NP, COVID-19 Nucleocapsid Protein, COVID-19 Nucleoprotein, SARS-CoV-2 NP, SARS-CoV-2 Nucleoprotein, Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein. Purity: >90% estimated by SDS-PAGE. Amino Acids: 1-419 (full length). MW: 49.7 kDa. Storage Buffer: 50 mM Tris-HCl, pH 7.4, with 150 mM sodium chloride and 0.1 M glycine.