SARS-CoV-2 nsp7 (recombinant)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. SARS-CoV-2 non-structural protein 7 (nsp7) is encoded within ORF1ab and is a cofactor in the RNA replication transcriptional complex. nsp7 is highly conserved in coronaviruses, and in SARS-CoV-2 it forms a decameric RNA replication transcriptional complex with the RNA polymerase cofactor nsp8 and the RNA-dependent RNA polymerase (RdRp) nsp12. Mutations in the nsp7-nps8 binding interface reduce the stability of the RNA replication transcriptional complex, leading to impaired RNA polymerase activity.Synonyms: SARS-CoV-2 Non-structural Protein 7, Severe Acute Respiratory Syndrome Coronavirus 2 nsp7. Purity: >90%. Endotoxin Testing: < 1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant SARS-CoV-2 C-terminal His-tagged nsp7 expressed in E. coli. Amino Acids: 3,831-3,913. Formulation: (Request formulation change), 50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride and 20% glycerol. UniProt Accession #: P0DTD1.