SARS-CoV-2 nsp14 Methyltransferase (recombinant)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. SARS-CoV-2 non-structural protein 14 (nsp14), also known as guanine-N7 methyltransferase (guanine-N7 MTase), is encoded within ORF1ab with an amino acid sequence that displays few mutations and is highly conserved across coronaviruses and SARS-CoV-2 variants. It is a bifunctional enzyme having an N-terminal exoribonuclease domain (ExoN domain) and a C-terminal methyltransferase domain. SARS-CoV-2 nsp14 forms an exonuclease complex with the cofactor nsp10, which binds to and stabilizes the disordered ExoN domain and is a necessary interaction for accurate exoribonuclease and RNA strand proofreading activities. It also forms a ternary methylation complex with nsp10 and nsp16 methyltransferase, also known as 2'-O-methyltransferase (2'-O-MTase), and catalyzes guanosine methylation in the viral mRNA 5'-end cap prior to the terminal ribose methylation catalyzed by 2'-O-MTase. SARS-CoV-2 nsp14 inhibits IFN-beta reporter gene activation induced by the retinoic acid-inducible gene I caspase activation and recruitment domain (RIG-I 2CARD domain) in HEK293FT cells, as well as inhibits global protein translation and expression of IFN-stimulated genes (ISGs) in HEK293T cells expressing SARS-CoV-2 nsp14.Synonyms: SARS-CoV-2 Guanine-N7 Methyltransferase, SARS-CoV-2 Guanine-N7 MTase, SARS-CoV-2 Non-structural Protein 14, Severe Acute Respiratory Syndrome Coronavirus 2 nsp14 Methyltransferase. Purity: >90% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Recombinant SARS-CoV-2 N-terminal GST-tagged nsp14 expressed in E. coli. Amino Acids: 1-527. Formulation: (Request formulation change), 50 mM Tris, pH 7.5, with 200 mM sodium chloride and 20% glycerol. UniProt Accession No: P0DTD1.