Sal-like protein 2 (SALL2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 1-198aa. Protein Length: Partial. Tag Info: N-terminal Flag-Myc-tagged. Target Protein Sequence: QTNTKATGKC NPNLHYWTAQ EQHNAAGIAW IPYFGPGAEG IYTEGLMHNQ NALVCGLRQL ANETTQALQL FLRATTELRT YTILNRKAID FLLRRWGGTC RILGPDCCIE PHDWTKNITD KINQIIHDFI DNPLPN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probable transcription factor that plays a role in eye development before, during, and after optic fissure closure. Reference: "Mutation of SALL2 causes recessive ocular coloboma in humans and mice."Kelberman D., Islam L., Lakowski J., Bacchelli C., Chanudet E., Lescai F., Patel A., Stupka E., Buck A., Wolf S., Beales P.L., Jacques T.S., Bitner-Glindzicz M., Liasis A., Lehmann O.J., Kohlhase J., Nischal K.K., Sowden J.C.Hum. Mol. Genet. 23:2511-2526(2014). Function: Probable transcription factor that plays a role in eye development before, during, and after optic fissure closure.