S-Catechol O-Methyltransferase (human, recombinant)

S-Catechol-O-methyltransferase (S-COMT) is the soluble cytosolic form of COMT. A rough endoplasmic reticulum membrane-bound form of COMT (MB-COMT) is also encoded by the COMT gene but produced via a separate promoter and contains an additional 50 amino acid residues, which compose the membrane anchor region. COMT is comprised of one domain with eight alpha helices surrounding a central beta sheet. It is the methyltransferase responsible for transferring a methyl group from S-adenosyl-L-methionine (SAM) to a catechol hydroxyl in the inactivation or degradation of catechol-containing molecules such as catecholamines. S-COMT is involved primarily in the inactivation of endogenous and xenobiotic catechols, such as catechol-containing hormones and carcinogenic flavonoids, and MB-COMT is involved primarily in the degradation of endogenous catecholamine neurotransmitters. S-COMT is the predominant form of COMT and is found primarily in the liver and kidney but is also found in the stomach, small intestine, adrenal gland, spleen, and brain. A valine-to-methionine substitution (Val158Met) in COMT affects its thermostability and may reduce its activity by greater than 50% in the human brain. The Val158Met variant of COMT is associated with Parkinson's disease in an ethnicity-dependent manner. COMT interacts with severe acute respiratory coronavirus 2 (SARS-CoV-2) non-structural protein 7 (Nsp7), which, together with Nsp8, forms the primase complex of the replicase-transcriptase complex of SARS-CoV-2. Cayman's S-Catechol-O-Methyltransferase (human, recombinant) protein can be used for ELISA, Western blot (WB), and enzyme activity assay applications.Synonyms: S-COMT, Pyrocatechol-O-methyltransferase, S-Adenosyl-L-methionine:Catechol O-Methyltransferase. Purity: >70% estimated by SDS-PAGE. Source: Active recombinant N-terminal His and SUMOpro tagged protein expressed in E. coli. Amino Acids: 2-221 (full-length). MW: 37.6 kDa. Formulation: (Request formulation change), 50 mM Hepes, pH 8.0, with 500 mM NaCl and 10% glycerol. UniProt Accession #: P21964.