Receptor-interacting serine/threonine-protein kinase 3 (Ripk3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 1-486aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSSVKLWPTG ASAVPLVSRE ELKKLEFVGK GGFGVVFRAH HRTWNHDVAV KIVNSKKISW EVKAMVNLRN ENVLLLLGVT EDLQWDFVSG QALVTRFMEN GSLAGLLQPE CPRPWPLLCR LLQEVVLGMC YLHSLNPPLL HRDLKPSNIL LDPELHAKLA DFGLSTFQGG SQSGSGSGSG SRDSGGTLAY LDPELLFDVN LKASKASDVY SFGILVWAVL AGREAELVDK TSLIRETVCD RQSRPPLTEL PPGSPETPGL EKLKELMIHC WGSQSENRPS FQDCEPKTNE VYNLVKDKVD AAVSEVKHYL SQHRSSGRNL SAREPSQRGT EMDCPRETMV SKMLDRLHLE EPSGPVPGKC PERQAQDTSV GPATPARTSS DPVAGTPQIP HTLPFRGTTP GPVFTETPGP HPQRNQGDGR HGTPWYPWTP PNPMTGPPAL VFNNCSEVQI GNYNSLVAPP RTTASSSAKY DQAQFGRGRG WQPFHK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family mbers. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. Reference: Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB.Pazdernik N.J., Donner D.B., Goebl M.G., Harrington M.A.Mol. Cell. Biol. 19:6500-6508(1999). Function: Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes