Pyruvate carboxylase 1 (PYC1), partial, Saccharomyces cerevisiae, recombinant

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 18-470aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EKNKILVANR GEIPIRIFRT AHELSMQTVA IYSHEDRLST HKQKADEAYV IGEVGQYTPV GAYLAIDEII SIAQKHQVDF IHPGYGFLSE NSEFADKVVK AGITWIGPPA EVIDSVGDKV SARNLAAKAN VPTVPGTPGP IETVEEALDF VNEYGYPVII KAAFGGGGRG MRVVREGDDV ADAFQRATSE ARTAFGNGTC FVERFLDKPK HIEVQLLADN HGNVVHLFER DCSVQRRHQK VVEVAPAKTL PREVRDAILT DAVKLAKECG YRNAGTAEFL VDNQNRHYFI EINPRIQVEH TITEEITGID IVAAQIQIAA GASLPQLGLF QDKITTRGFA IQCRITTEDP AKNFQPDTGR IEVYRSAGGN GVRLDGGNAY AGTIISPHYD SMLVKCSCSG STYEIVRRKM IRALIEFRIR GVKTNIPFLL TLLTNPVFIE GTYWTTFIDD TPQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Reference: Global analysis of protein expression in yeast.Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.Nature 425:737-741(2003). Function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.