Protein Dihydroorotate dehydrogenase (quinone), mitochondrial(Dhodh), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 11-395aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: LDAAIILGGG GLLFTSYLTA TGDDHFYAEY LMPALQRLLD PESAHRLAVR VISLGLLPRA TFQDSNMLEV RVLGHKFRNP VGIAAGFDKH GEAVDGLYKL GFGFVEVGSV TPQPQEGNPR PRVFRLPEDQ AVINRYGFNS HGLSAVEHRL RARQQKQTQL TTDGLPLGIN LGKNKTSVDA AADYVEGVRI LGPLADYLVV NVSSPNTAGL RSLQGKTELR RLLSKVLQER DALKGPQKPA VLVKIAPDLT AQDKEDIASV ARELGIDGLI ITNTTVSRPV GLQGALRSET GGLSGKPLRD LSTQTIREMY ALTQGTIPII GVGGVSSGQD ALEKIQAGAS LVQLYTALTF LGPPVVARVK RELEALLKER GFNTVTDAIG VDHRR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Reference: "In vitro and in Vivo inhibition of immunoglobulin secretion by the immunosuppressive compound HR325 is reversed by exogenous uridine." Thomson T.A., Spinella-Jaegle S., Francesconi E., Meakin C., Millet S., Flao K.L., Hidden H., Ruuth E. Scand. J. Immunol. 56:35-42(2002). Function: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.