Prostaglandin E2 receptor EP4 subtype (Ptger4), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 361-513aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA MSGHSRSFLA RELKEISSTS QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ ADTTSLRTLR ISETSDSSQG QDSESVLLVD EVSGSHREEP ASKGNSLQVT FPSETLKLSE KCI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Reference: "Prostaglandin E receptor type 4-associated protein interacts directly with NF-kappaB1 and attenuates macrophage activation." Minami M., Shimizu K., Okamoto Y., Folco E., Ilasaca M.L., Feinberg M.W., Aikawa M., Libby P. J. Biol. Chem. 283:9692-9703(2008). Function: nan