Potassium-transporting ATPase subunit beta (ATP4B), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 58-291aa. Protein Length: Extracellular Domain. Tag Info: Tag-Free. Target Protein Sequence: CLYVLMQTVD PYTPDYQDQL RSPGVTLRPD VYGEKGLEIV YNVSDNRTWA DLTQTLHAFL AGYSPAAQED SINCTSEQYF FQESFRAPNH TKFSCKFTAD MLQNCSGLAD PNFGFEEGKP CFIIKMNRIV KFLPSNGSAP RVDCAFLDQP RELGQPLQVK YYPPNGTFSL HYFPYYGKKA QPHYSNPLVA AKLLNIPRNA EVAIVCKVMA EHVTFNNPHD PYEGKVEFKL KIEK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity. Reference: "A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules." Bab-Dinitz E., Albeck S., Peleg Y., Brumfeld V., Gottschalk K.E., Karlish S.J. Biochemistry 48:8684-8691(2009). Function: Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.