Poly [ADP-ribose] polymerase tankyrase-2 (TNKS2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 57-776aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: RKSTPLHFAA GFGRKDVVEY LLQNGANVQA RDDGGLIPLH NACSFGHAEV VNLLLRHGAD PNARDNWNYT PLHEAAIKGK IDVCIVLLQH GAEPTIRNTD GRTALDLADP SAKAVLTGEY KKDELLESAR SGNEEKMMAL LTPLNVNCHA SDGRKSTPLH LAAGYNRVKI VQLLLQHGAD VHAKDKGDLV PLHNACSYGH YEVTELLVKH GACVNAMDLW QFTPLHEAAS KNRVEVCSLL LSYGADPTLL NCHNKSAIDL APTPQLKERL AYEFKGHSLL QAAREADVTR IKKHLSLEMV NFKHPQTHET ALHCAAASPY PKRKQICELL LRKGANINEK TKEFLTPLHV ASEKAHNDVV EVVVKHEAKV NALDNLGQTS LHRAAYCGHL QTCRLLLSYG CDPNIISLQG FTALQMGNEN VQQLLQEGIS LGNSEADRQL LEAAKAGDVE TVKKLCTVQS VNCRDIEGRQ STPLHFAAGY NRVSVVEYLL QHGADVHAKD KGGLVPLHNA CSYGHYEVAE LLVKHGAVVN VADLWKFTPL HEAAAKGKYE ICKLLLQHGA DPTKKNRDGN TPLDLVKDGD TDIQDLLRGD AALLDAAKKG CLARVKKLSS PDNVNCRDTQ GRHSTPLHLA AGYNNLEVAE YLLQHGADVN AQDKGGLIPL HNAASYGHVD VAALLIKYNA CVNATDKWAF TPLHEAAQKG RTQLCALLLA HGADPTLKNQ. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity. Reference: n/a. Function: nan