Platelet-activating factor acetylhydrolase (Pla2g7), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 22-440aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: FHWQDTSSFD FRPSVMFHKL QSVMSAAGSG HSKIPKGNGS YPVGCTDLMF GYGNESVFVR LYYPAQDQGR LDTVWIPNKE YFLGLSIFLG TPSIVGNILH LLYGSLTTPA SWNSPLRTGE KYPLIVFSHG LGAFRTIYSA IGIGLASNGF IVATVEHRDR SASATYFFED QVAAKVENRS WLYLRKVKQE ESESVRKEQV QQRAIECSRA LSAILDIEHG DPKENVLGSA FDMKQLKDAI DETKIALMGH SFGGATVLQA LSEDQRFRCG VALDPWMYPV NEELYSRTLQ PLLFINSAKF QTPKDIAKMK KFYQPDKERK MITIKGSVHQ NFDDFTFVTG KIIGNKLTLK GEIDSRVAID LTNKASMAFL QKHLGLQKDF DQWDPLVEGD DENLIPGSPF DAVTQVPAQQ HSPGSQTQN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Reference: "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad." Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W. J. Biol. Chem. 270:25481-25487(1995). Function: nan